Distinct Patterns of Gene and Protein Expression Elicited by Organophosphorus Pesticides in Caenorhabditis elegans

Abstract

The wide use of organophosphorus (OP) pesticides makes them an important public health concern. Persistent effects of exposure and the mechanism of neuronal degeneration are continuing issues in OP toxicology. To elucidate early steps in the mechanisms of OP toxicity, we studied alterations in global gene and protein expression in Caenorhabditis elegans exposed to OPs using microarrays and mass spectrometry. We tested two structurally distinct OPs (dichlorvos and fenamiphos) and employed a mechanistically different third neurotoxicant, mefloquine, as an outgroup for analysis. Treatment levels used concentrations of chemical sufficient to prevent the development of 10%, 50% or 90% of mid-vulval L4 larvae into early gravid adults (EGA) at 24 h after exposure in a defined, bacteria-free medium.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2009
Accession Number
ADA524755

Entities

People

  • David A. Jackson
  • Elizabeth Gehman
  • John A. Lewis
  • Maria Szilagyi
  • William E. Dennis

Organizations

  • U.S. Army Center for Environmental Health Research

Tags

DTIC Thesaurus Topics

  • Biological Sciences
  • Biomedical Research
  • Cell Physiological Processes
  • Cells
  • Chemical Analysis
  • Chemistry
  • Computational Science
  • Data Analysis
  • Liquid Chromatography
  • Mass Spectrometry
  • Pesticides
  • Proteins
  • Proteomics
  • Public Health
  • Spectrometry
  • Supervised Machine Learning
  • Toxicity

Readers

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