Investigation of Antiangiogenic Mechanisms Using Novel Imaging Techniques
Abstract
This study is intended to investigate the physiological effects of antiangiogenic treatment at the microcirculatory level with the intent to better understand how such modulations of the tumor environment can sensitize the tumor to conventional cytotoxic therapies. To this end, we employ the window chamber model to optically observe natural and induced changes in tumor physiology. A number of advanced imaging modalities have been adapted to this model in order to facilitate longitudinal, in vivo investigation into the parameters of interest. These include Doppler Optical Coherence Tomography for the measurement of blood flow velocity, Hyperspectral Imaging for the measurement of hemoglobin saturation and hematocrit, and Confocal Imaging for the measurement of 3-D vascular architecture and perfusion. Additionally, novel methods have been developed to molecularly probe the tumor environment. These include Oxygen Sensitive Nanoparticles for the mapping of pO2 and the use of Engineered Cell Lines which express green fluorescent protein under hypoxia. Together, these techniques will allow us to better understand and model the process of tumor growth and response to treatment, eventually leading to a better understanding of how such changes can be exploited to increase the efficacy of combinational therapies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2010
- Accession Number
- ADA524932
Entities
People
- Andrew Fontanella
Organizations
- Duke University Hospital