Role of Fetuin-A in Breast Tumor Cell Growth

Abstract

In this final report we report that we successfully completed task # 1, after doing additional experiments requested by the reviewers. The manuscript is presently under editorial review in the American Journal of Pathology. In the past year we have concentrated mainly on Task # 2. The most significant reportable outcome of this task is that we have now removed doubts regarding the authenticity of fetuin-A as adhesion and growth signaling molecule. The purification protocol we have developed can purify fetuin-A to 100% purity. More importantly we have shown that human fetuin-A (ahsg) behaves exactly like its bovine counterpart. We have shown that in addition to mediating the activation of PI3 kinase/Akt, fetuin-A at high concentrations can also activate MAP kinases. We have also shown that in regular tissue culture, fetuin-A could be more relevant for cellular attachment than integrins. Fetuin-A is also actively internalized by the tumor cells but the significance of this internalization is presently unclear.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2010
Accession Number
ADA525086

Entities

People

  • Josiah Ochieng

Organizations

  • Meharry Medical College

Tags

DTIC Thesaurus Topics

  • Adhesion
  • Alpha Globulin
  • Antibodies
  • Attachment
  • Biomedical Research
  • Blood Proteins
  • Breast Cancer
  • Cell Line
  • Cells
  • Department Of Defense
  • Epithelial Cells
  • Glycerols
  • Molecules
  • Neoplasms
  • Pathology
  • Proteins
  • Tissue Culture

Readers

  • Breast cancer cell signaling and growth regulation.
  • Technical Research and Report Writing.