Multidisciplinary Analysis of Cyclophilin A Function in Human Breast Cancer

Abstract

The ongoing purpose of this work is to examine the effects of altered cyclophilin A (CypA) levels and activity on the prolactin receptor (PRLr) associated signaling and its role in breast cancer development and/or progression. To this end, we have further demonstrated that siRNA-mediated CypA knockdown or the use of CypA inhibitors such as cyclosphorine A (CsA) analogs-CsD and CsH significantly abrogated the PRLr signaling and breast cancer cell growth in vitro. In addition, by using a novel antibody phage display technology, we have developed several synthetic antibodies, which exhibit nanomolar affinities for the CypA. Experiments are currently underway to test these potential synthetic antibodies in vitro and in vivo.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2010
Accession Number
ADA525263

Entities

People

  • Jiamao Zheng

Organizations

  • Northwestern University

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Antibodies
  • Biological Sciences
  • Breast Cancer
  • Chemistry
  • Department Of Defense
  • Gene Expression
  • Inhibitors
  • Molecules
  • Neoplasms
  • Peptide Growth Factors
  • Peptides
  • Proteins
  • Recombinant Proteins
  • Schematic Diagrams
  • Surface Plasmon Resonance
  • Three Dimensional

Fields of Study

  • Biology
  • Chemistry

Readers

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