A Mouse Model to Investigate Postmenopausal Biology as an Etiology of Ovarian Cancer Risk

Abstract

We are completing this project to use a germ cell deficient Wv mouse model to test the hypothesis of a synergy between oncogenic mutations and postmenopausal biology in ovarian cancer development. We found that crossing of Wv mice into mutant p53 or pten (+/-) background did not lead to a malignant tumor phenotype (Aim 1). Instead, the mutants rescue ovarian germ cells, a very interesting finding. The ovarian surface epithelia in Wv/Wv:p27 (+/-) or Wv/Wv:p27 (+/-) compound mutant mice develop unique lesions with peculiar morphology and formed large ovarian tumors in older mice (Aim 2). The analysis of tumor phenotypes (Aim 3) is ongoing and we hope to complete and report the findings in next several months. Thus, in this project we have successfully developed Wv/Wv:p27 (+/-) mice as suitable models of ovarian epithelial cancer. We conclude that the result support our hypothesis that the collaboration of reproductive factors and genetic mutations leads to the development of ovarian cancer. The study also provides us with future directions, and we plan to seek future support to use flox-p53 mutant mice to create additional models. In sum, the project is completed as planned and is successful, and provides basis for further advance.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 2009
Accession Number
ADA525609

Entities

People

  • Xiangxi Xu

Organizations

  • University of Miami

Tags

DTIC Thesaurus Topics

  • Alkanes
  • Biology
  • Cancer
  • Cell Line
  • Cells
  • Chemistry
  • Culture Techniques
  • Genes
  • Genetics
  • Genome
  • Germ Cells
  • Neoplasms
  • Ovarian Cancer
  • Proteins
  • Surgery
  • Tissues
  • Viruses

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Oncology (Cancer Research).
  • Regression Analysis.

Technology Areas

  • Biotechnology