Activation of Estrogen Receptor-alpha by Novel Anions

Abstract

The overall purpose of my project is to investigate the direct interaction of ER-alpha with eNOS and nitrite, and to determine what roles they might play in hormone independent breast cancers. I hope to characterize the interaction between ER-alpha and eNOS in the absence and presence of growth factors, determine whether polymorphic forms of eNOS increase ER-alpha activity due to increased nitrite production, and to determine the mechanism by which nitrite activates ER-alpha. Important findings to date have shown that treatment of wild-type ERalpha with nitrite leads to the dissociation of the hsp90 complex, binding to DNA, and the recruitment of the coactivator SRC-1 and RNA polymerase II to estrogen regulated gene promoters. These findings provide a novel role for nitrite in activation of ER-alpha.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2010
Accession Number
ADA527159

Entities

People

  • Geoffrey Storchan
  • Mary B. Martin

Organizations

  • Georgetown University

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Amino Acids
  • Antibodies
  • Biomedical Research
  • Biomolecules
  • Breast Cancer
  • Cells
  • Chemical Reactions
  • Dissociation
  • Estrogens
  • Growth Factors
  • Hormones
  • Neoplasms
  • Polymerase Chain Reaction
  • Sodium Compounds
  • Tumor Cell Line
  • Universities

Readers

  • Aquatic Ecology
  • Breast cancer cell signaling and growth regulation.
  • Molecular Genetics