Human Neural Cell-Based Biosensor
Abstract
Human neural progenitor cells have a strong potential for use as cell-based biosensors for environmental toxins. The overall goal of this project is to develop a human neural cell based biosensor using ArunA's neural progenitor cell line, hNP1-tm. In this report, we detail the following progress toward development of a human cell-based biosensor using these neural progenitor cells: (1) we continue to develop methods for enriching neural progenitor populations using VVA lectin and antibodies against CNTFR-alpha, (2) we have begun to develop methods for directed differentiation of neural progenitors into motoneurons and astrocytes using defined medium conditions, and (3) we have begun development of fluorescence based assays for proliferation, mitochondrial function and reactive oxygen species generation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 10, 2010
- Accession Number
- ADA528307
Entities
People
- Allan Powe
- Jamie Chilton
- Steven L. Stice