Methods for Imaging Shewanella Oneidensis MR-1 Nanofilaments

Abstract

Nanofilament production by Shewanella oneidensis MR-1 was evaluated as a function of lifestyle (planktonic vs. sessile) under aerobic and anaerobic conditions using different sample preparation techniques prior to imaging with scanning electron microscopy. Nanofilaments could be imaged on MR-1 cells grown in biofilms or planktonically under both aerobic and anaerobic batch culture conditions after fixation, critical point drying and coating with a conductive metal. Critical point drying was a requirement for imaging nanofilaments attached to planktonically grown MR-1 cells, but not for cells grown in a biofilm. Techniques described in this paper cannot be used to differentiate nanowires from pili or flagella.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2010
Accession Number
ADA529088

Entities

People

  • B. R. Ringeisen
  • Bryan Little
  • L. A. Fitzgerald
  • R. Ray
  • S. Lizewski

Organizations

  • United States Naval Research Laboratory

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Bacteria
  • Cells
  • Cellular Structures
  • Chemistry
  • Electron Acceptors
  • Electron Microscopes
  • Electron Microscopy
  • Electron Transfer
  • Electrons
  • Escherichia Coli
  • Gram-Negative Bacteria
  • Materials
  • Microorganisms
  • Microscopy
  • Nanowires
  • Production
  • Scanning Electron Microscopy

Readers

  • Medical Imaging.
  • Microbial Pathology
  • Nanoscale Plasmonic Nanotechnology

Technology Areas

  • Biotechnology
  • Biotechnology - Bioremediation
  • Microelectronics
  • Microelectronics - Graphene