The Use of Comprehensive Molecular Profiling With Network and Control Theory to Better Understand GWI and Model Therapeutic Strategies
Abstract
The objective of this study is to improve our understanding of GWI pathogenesis in two ways through integration across several of the body's regulatory systems of data and knowledge collected from disparate sources, and by mapping of the coordinated interactions between these physiologic systems and the potential for altered "wiring" of these signaling networks in GWI. Using comprehensive molecular profiling, network and control theory the overarching objective of this proposal is to define the precise nature of these irregularities in immune and neuroendocrine signaling as well as the altered activation states of the corresponding cells such that treatment courses can be designed to redirect the system as a whole to normal pattern of coordinated activity. Active recruitment is under way and seven patients have been consented to participate in the study. Four of the patients are symptomatic with GWI and three are healthy controls. There was a delay in initiating the exercise challenge component of the study due to local requirements, documenting and approvals through several committees on the standard operating procedures for the sterilization of our reusable equipment which consist of a mask attached to the equipment that measures expired gases. The sterilization process was approved by the Infectious Control Committee and the Reusable Medical Equipment committee as of June 23, 2010 and Exercise Challenges will commence the first week of August. The laboratory measures have been standardized and validated. The analytic plan is being refined, and our co-investigator, Dr Broderick is working with the preliminary data to develop the best method to utilize the data from the preliminary study to validate the findings of the current study. The study, involving a short exercise challenge and serial blood draws, is a single point in time study, without an intervention.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2010
- Accession Number
- ADA529400
Entities
People
- Mary A. Fletcher
- Nancy G. Klimas
Organizations
- Veterans Administration Medical Center