Assessment of Regenerative Capacity in the Dolphin

Abstract

Described herein is the technical information pertaining to Year 1 of a multi-year effort to determine and characterize the use of adipose-derived (fat-derived) stem cells in the treatment of epidermal (skin) wounds. Adipose tissue was successfully harvested from the nuchal fat pad of six Atlantic Bottlenose dolphins via liposuction; cells released during the digestion of the adipose tissue were analyzed for cytology, assayed for the total number of colony-forming cells, expanded in culture, differentiated into multiple cell lineages and analyzed for stem cell surface markers. Cultured cells were also cryogenically frozen for future cell therapy treatment of dolphin skin wounds.

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Document Details

Document Type
Technical Report
Publication Date
Oct 10, 2010
Accession Number
ADA530626

Entities

People

  • Jeffrey M. Catania
  • Robert J. Harman

Tags

Communities of Interest

  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Adipose Tissue
  • Bariatrics
  • Blood
  • Blood Cells
  • Cell Count
  • Cell Lineage
  • Cells
  • Connective Tissue
  • Culture Techniques
  • Cultured Cells
  • Digestive System Processes
  • Leukocytes
  • Marine Mammals
  • Medical Personnel
  • Stem Cells
  • Therapy
  • Tissues

Readers

  • Marine Mammal Biology
  • Molecular Biology and Genetics
  • Trauma Surgery or Emergency Medicine.

Technology Areas

  • Biotechnology