A Polyamine Oxidizing Enzyme as a Drug to Treat Breast Cancer
Abstract
Work was initiated to test two polyethylene glycolated (PEGylated) forms of bovine serum amine oxidase (SAO) as effective treatments for breast cancer using a mouse model. Hopefully, this approach, or a variation thereof, could be used as a new a therapy for breast and other human cancers. Currently, a large quantity of pure bovine SAO is in hand, which was obtained from ~ 10 gallons of fresh cow blood. A final purification step was used to produce large quantities of extremely pure SAO. Attempts to find a cost-effective, practical method for the degycosylation of SAO were unsuccessful. Using different PEGylating reagents, we attempted to obtain several PEG derivatives of SAO. Although preliminary test indicated that SAO could be PEGylated at a fairly high level, efforts to repeat this on a large scale failed. Hence, it has not been possible to test any PEG-SAO derivatives in non-tumorigenic mice, or mice harboring human breast-cancer tumors. Effort to find efficient ways to PEGylate SAO at a high level will continue. High level PEGylation occurred with freshly prepared SAO. Although about 3 grams of highly pure enzyme, it is proposed that freshly prepared oxidase is required. In the future, will carry out smaller scale purifications, and only use the freshly prepared SAO for PEGylation, & we will try different PEGylation reagents. Hopefully, the problem can be remedied.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2009
- Accession Number
- ADA532320
Entities
People
- William S. Mcintire
Organizations
- Northern California Institute for Research and Education