In Utero Influences, Breast Stem Cells, and Breast Cancer Risk Factors

Abstract

Breast density can be assessed by whole mount carmine alum staining of the fourth inguinal mammary glands according to the method reported by Brisken et al (2002) and counting the number of terminal end buds. Mammary glands of pregnant C57Bl/6J mice were found to have significantly more terminal end buds than virgin mice. Flow cytometric analyses of dissociated cells from mammary glands clearly identified a sub-population of cells that are CD49f + and CD24+, putative markers of mammary stem/progenitor cells.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2009
Accession Number
ADA532382

Entities

People

  • Chung-cheng Hsieh

Organizations

  • University of Massachusetts

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Anatomy
  • Biological Sciences
  • Biological Staining And Labeling
  • Biomedical Research
  • Body Weight
  • Breast Cancer
  • Cells
  • Demographic Cohorts
  • Department Of Defense
  • Glands
  • Growth Factors
  • Mammary Glands
  • Neoplasms
  • Risk Factors
  • Stem Cells
  • Terminals

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Women's Health and Cancer Risk Research: African American Women and Pregnancy Outcomes.

Technology Areas

  • Biotechnology