Dual Delivery of Growth Factors and or Antibiotics from Chitosan-Composites for Bone Regeneration
Abstract
This one year project investigated (in vitro) the ability of microsphere-based chitosan-calcium sulfate composites to locally deliver growth factor and antibiotics to heal bone and prevent infection in traumatic musculoskeletal injuries. Antibiotic, vancomycin (vanc) was loaded into calcium sulfate (CaS) and microspheres loaded with either vanc or bone morphogenetic protein 2 (BMP-2). In vitro release was evaluated over 6 weeks and biological activity and interactions of released factors were evaluated in cultures. Composites released vanc at levels (>40microg/ml) that killed S. aureus for 18 days. BMP-2 release from microspheres was reduced to ~1ng/ml/day when combined with CaS as compared to plain microspheres (~9ng/ml/day for days 6-10 elution). This may be due to high affinity of BMP for calcium and hence may serve to retain BMP in wound site. There was no overt toxicity with levels of vanc (<3600microg/ml) and BMP-2 released, but the presence of vanc suppressed (~20-50%) BMP-2 induced alkaline phosphatase expression in cells. Additional work will need to determine retention of BMP by composite and long term effects of vancomycin on BMP-2 stimulated osteogenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2010
- Accession Number
- ADA532903
Entities
People
- Joel D. Bumgardner
Organizations
- University of Memphis