Human Neural Cell-Based Biosensor
Abstract
Human neural progenitor cells have a strong potential for use as cell-based biosensors for environmental toxins. The overall goal of this project is to develop a human neural cell based biosensor using ArunA's neural progenitor cell line, hNP1(trademark). In this report, we detail the progress in development for the following areas: (1) methods to isolate and enrich for neural progenitors using cell surface markers, (2) methods to directed differentiation of progenitors into dopaminergic neurons, motoneurons and astrocytes using defined medium conditions, (3) development of cell-based methods to detect botulinum toxin, and (4) development of fluorescence based assays for proliferation, mitochondrial function and reactive oxygen species generation as sensor elements.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 10, 2010
- Accession Number
- ADA533672
Entities
People
- Allan Powe
- Jamie Chilton
- Steven L. Stice