Defining Genomic Changes in Triple Negative Breast Cancer in Women of African Descent

Abstract

Breast cancer (BC) is the second leading cause of cancer death among African-American (AA) women, with mortality 20% greater than that in Caucasians (Cauc). However, the basis for such disparity remains an enigma. Recent observations from our laboratory suggest the involvement of unidentified genes contributing to AA BC risk. Matched tumor and normal FFPE samples from Cauc and AA patients were obtained from the UM /Sylvester Breast Tissue Bank (UM/S BTB) under an IRB-approved protocol. Based on analysis of 22,000 transcripts, ethnic specific gene expression patterns were identified that may provide important new insights into molecular mechanisms of ethnic subtype differences in clinical outcomes. We propose to extend these preliminary findings to a large African tumor bank [available via collaboration between Drs. Peter A. Bird (Kijabe, Kenya) and Mark Pegram (UM Sylvester).] Additionally, we propose to analyze chromosomal alterations associated with gene expression differences utilizing array cGH (in collaboration with Alan Ashworth, England). This work will contribute to development of rationale designs of preventive, predictive and therapeutic measures for BC in different ethnicities, and thus, a significant reduction in current ethnicspecific disparities in BC incidence, morbidity and mortality. Hypothesis: Discrete genomic alterations and gene expression changes will be identified and shared between triple negative tumor specimens within an ethnic group, i.e., North Americans/African decent and Kenya. Aim I: Analyze and compare genome-wide differences in gene expression in BC samples of AA ancestry vs. native African (Kijabe) samples (Drs. Pegram, Baumbach, Bird, Halsey). Aim II: Investigate possible chromosomal alterations associated with gene expression differences (Drs. Pegram, Baumbach, Ashworth). Aim III: Analyze ancestry of each sample using a panel of ancestry-informative DNA markers (Drs. Kittles, Baumbach). Synergy Statement.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2010
Accession Number
ADA533680

Entities

People

  • Lisa Baumbach
  • Mark Pegman

Organizations

  • University of Miami

Tags

DTIC Thesaurus Topics

  • African Americans
  • Breast Cancer
  • Caucasians
  • Cell Physiological Processes
  • Data Analysis
  • Data Science
  • Detection
  • Diseases And Disorders
  • Ethnic Groups
  • Factor Analysis
  • Gene Expression
  • Genetics
  • Information Science
  • Neoplasms
  • Regression Analysis
  • Statistical Analysis
  • Two Dimensional

Readers

  • Molecular and genetic basis of cancer.
  • Neurological Diseases/Conditions/Disorders
  • Women's Health and Cancer Risk Research: African American Women and Pregnancy Outcomes.