Enhancing Natural Attenuation through Bioaugmentation with Aerobic Bacteria that Degrade cis-1,2-Dichloroethene
Abstract
Monitored natural attenuation (MNA) and enhanced in situ bioremediation (EISB) remedies hold the promise of reducing the costs associated with the cleanup of Department of Defense (DoD) sites impacted by chlorinated solvents. However, there are many DoD sites where tetrachloroethene (PCE) and trichloroethene (TCE) are undergoing only partial dechlorination to cis-1,2-dichloroethene (cDCE), even when sufficient electron donor is present, either because of the absence of required bacteria (Dehalococcoides) or aerobic conditions. Under sponsorship from the Strategic Environmental Research and Development Program (SERDP) (Project ER-1168), a novel aerobic bacterium (Polaromonas sp. strain JS666) that uses cDCE as a sole carbon and energy source was isolated and characterized (Coleman et al., 2002a,b). Since it requires no exotic growth factors, JS666 is a promising bioaugmentation culture for aerobic sites where cDCE is recalcitrant. The microorganism will grow and thrive where oxygen and cDCE are colocated, and JS666 also degrades 1,2-dichloroethane (DCA) and cometabolizes TCE and vinyl chloride (VC). Ideal groundwater conditions for JS666 include dissolved oxygen (DO) levels between 0.01 milligrams per liter (mg/L) and 8 mg/L; low ionic strength (conductivity <15 millisiemens per centimeter [mS/cm]); a pH of 6.5 to 8; and relatively low concentrations of TCE, 1,2-DCA, and VC (<500 micrograms per liter [micrograms/L]).
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2010
- Accession Number
- ADA534196
Entities
Organizations
- Environmental Security Technology Certification Program