Optimization of Antibody-Conjugated Magnetic Nanoparticles for Target Preconcentration and Immunoassays

Abstract

Biosensors based on antibody recognition have a wide range of monitoring applications that apply to clinical environmental, homeland security, and food problems. In an effort to improve the limit of detection of the Naval Research Laboratory (NRL) Array Biosensor, magnetic nanoparticles (MNPs) were designed and tested using a fluorescence-based array biosensor. A full optimization study of the antibody-modified MNPs and their use in the biosensor was performed. The results demonstrate the impact of magnetic extraction using the MNPs labeled with fluorescent proteins both for target preconcentration and for subsequent integration into immunoassays performed under flow conditions for enhanced signal generation.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2010
Accession Number
ADA534282

Entities

People

  • Frances Ligler
  • Joshua E. Smith
  • Kim E. Sapsford
  • Tan Weihong

Organizations

  • United States Naval Research Laboratory

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Albumins
  • Biosensors
  • Charge Coupled Devices
  • Chemical Synthesis
  • Chemistry
  • Detection
  • Detectors
  • Digital Images
  • Fluorescence
  • Magnetic Nanoparticles
  • Magnetometers
  • Materials
  • Military Research
  • Molecules
  • Nanoparticles
  • Polymerase Chain Reaction
  • Polymers

Readers

  • Medical Imaging.
  • Nanocomposite Materials Science
  • Technical Research and Report Writing.

Technology Areas

  • Biotechnology