Using Simulated Microgravity to Enhance the Effectiveness of Nanodrug Chemotherapy in Breast Cancer
Abstract
The aim of this study is to define whether exposing mice bearing breast tumors to simulated microgravity will increase nanoparticle drug convection from the blood to the tumor tissue by modifying the balance in the Starling-Landis equation. In this two-month period, breast tumors were established in mice using human (MDA-MB-231) and mouse (PY8119) cell lines and conditions for measuring interstitial fluid pressure (IFP) and blood and tissue distribution of fluorescent-labeled dextrans were set up. In preliminary experiments, no significant differences in tumor IFP and dextran uptake were observed between groups of mice submitted or not to microgravity simulation. Overgrown tumors (> 250mm3) often presented necrosis and were more prone to broader variations in IFP and dextran uptake, hence adjustments to the procedure are necessary. An improved technique for interstitial fluid withdrawal was developed, allowing larger and cleaner yields of fluid. Capillary hydrostatic pressure measurement is the next step. The results obtained create the basis for the performance of a solid study.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2010
- Accession Number
- ADA534748
Entities
People
- Leonardo Carvalho