Complete Genome Sequence of Rothia mucilaginosa DY-18: A Clinical Isolate with Dense Meshwork-Like Structures from a Persistent Apical Periodontitis Lesion

Abstract

Rothia mucilaginosa is an opportunistic pathogen in the human oral cavity and pharynx. We found that R. mucilaginosa DY-18, a clinical isolate from a persistent apical periodontitis lesion, had biofilm-like structures. Similar structures were also observed on R. mucilaginosa ATCC25296. To further study these structures, we determined the complete genome sequence of DY-18 and found it a 2.26-Mb chromosome. Regarding stress responsive systems known to affect biofilm formation in many bacteria, DY-18 genome possessed only two sigma factor genes. One of these encoded an additional sigma factor whose promoter-binding activity may be regulated in response to environmental stimuli. Additionally, several genes assigned to two-component signal transduction systems were presented in this genome. To the best of our knowledge, this is the first complete genome of R. mucilaginosa species and our data raise the possibility that this organism regulates the biofilm phenotype through these stress responsive systems.

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Document Details

Document Type
Technical Report
Publication Date
Sep 25, 2010
Accession Number
ADA534768

Entities

People

  • Chicko Sugimori
  • Chiho Mashimo
  • Hisanori Fukushima
  • Kai-poon Leung
  • Kazuyoshi Yamane
  • Takayuki Nambu
  • Takeshi Yamanaka

Organizations

  • Osaka Dental University

Tags

Communities of Interest

  • Energy and Power Technologies

DTIC Thesaurus Topics

  • Amino Acids
  • Bacteria
  • Bacteriology
  • Cell Physiological Processes
  • Cells
  • Electron Microscopes
  • Electron Microscopy
  • Metabolism
  • Microbiology
  • Microbiomes
  • Microorganisms
  • Microscopes
  • Microscopy
  • Mouth Diseases
  • Production
  • Scanning Electron Microscopy
  • Sequences

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular Genetics
  • Spectroscopy.

Technology Areas

  • Biotechnology