Novel Aptamers to Target Metastasis
Abstract
The overall goal of this project is to develop novel method to inhibit cancer metastasis. The major hypothesis to be tested is that aptamers (short oligonucleotides) can be developed that target the process of invasion, without prior knowledge of a target protein, and that these aptamers will inhibit the development of metastasis. We have identified two aptamers (Mod 12 and Mod 13) using a novel application of a process called "systemic evolution of ligands by exponential enrichment" (SELEX), that bind PCa cells that we selected for their high invasive ability. In the work done to date, we have optimized aptamer synthesis for quality and quantity; identified that the aptamers work best on PCa, as opposed to other cancer cell types; identified that Mod 13 is more effective at inhibiting in vitro invasion then Mod 12; determined that AIAs have no direct impact on overall cell growth in vitro; and established a novel bioluminescent method to measure invasion of cells in vitro. These results now have has poised to be able to assess the ability of Mod 13 to inhibit PCa metastasis in an in vivo model in ongoing studies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2010
- Accession Number
- ADA534850
Entities
People
- Evan T Keller
Organizations
- University of Michigan