Myeloperoxidase in the Progression of Breast Cancer
Abstract
The main objective of this project is to establish whether myeloperoxidase (MPO) is protective in breast cancer. We will test this concept in mouse models of human breast cancer and predict that MPO will slow tumor growth, progression and metastasis. If MPO is protective, a likely mechanism is its role in macrophage-mediated killing of breast cancer cells. This idea will be also tested in this project. There are three Specific Aims: (1) To assess mammary tumor progression in polyoma middle T oncogene (PyMT) mice expressing human MPO (huMPO) or being mouse MPO (moMPO) deficient. (2) To determine the role of huMPO in the growth and metastasis of human breast tumors in mice. (3) To test whether huMPO produced by macrophages kills breast cancer cells. In this first year of funding we have set up the mouse colonies and breeder and are producing mice for the experiments. All methodologies, including immuno-histochemistry, PCR and qPCR detection of huMPO in mouse tissues have been established. Production of cell lines for further experiments is in progress. Initial results suggest that whereas tumor onset is not delayed in PyMT mice expressing huMPO compared to PyMT wt mice, tumor growth is attenuated. At the same time it appears that metastasis is not inhibited. These observations need to be confirmed using larger numbers of mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2010
- Accession Number
- ADA534961
Entities
People
- Barbara M. Mueller
Organizations
- Torrey Pines Institute for Molecular Studies