Effect of Interstitial Pressure on Epithelial Invasion from Human Mammary Ducts

Abstract

The objectives of this Concept Award are to develop methods to engineer open "ducts" of human mammary epithelial cells in collagen-based gels in vitro, and to study how the distinct pressures in the apical (lumenal) and basal sides of the epithelium affect tumor invasion. This work has yielded a technique to form ducts of immortalized MCF-10A human mammary epithelial cells; application of similar methods to primary cells was unsuccessful in creating stable ducts. Open ducts of MCF-10A cells could be exposed to different apical and basal pressures by interfacing the gels and the open ends of ducts to different liquid reservoirs. This work provides new tools for studying how physical forces affect the progression of human breast tumors in a well-controlled in vitro setting.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2010
Accession Number
ADA535675

Entities

People

  • Joe Tien

Organizations

  • Boston University

Tags

DTIC Thesaurus Topics

  • Acquisition
  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cells
  • Collagen
  • Department Of Defense
  • Diseases And Disorders
  • Enzyme Inhibitors
  • Epithelial Cells
  • Epithelium
  • Gene Expression
  • Hydrostatic Pressure
  • Information Operations
  • Inhibitors
  • Neoplasms

Fields of Study

  • Medicine

Readers

  • Combustion and Flow Dynamics.
  • Oncology (Cancer Research).
  • Trauma Surgery or Emergency Medicine.