Evaluation of Five Jet Fuels in the Salmonella-Escherichia coli / Microsome Plate Incorporation Assay
Abstract
Five jet fuels were evaluated for mutagenic activity in the Salmonella typhimurium-Escherichia coli/microsome plate incorporation assay. The assay was performed using the plate incorporation procedure with S. typhimurium strains TA1535, TA1537, TA98, and TA100 and E. coli strain WP2 (uvrA) in both the presence and absence of a metabolic activation (MA) mixture containing an Aroclor 1254 induced rat-liver S9. A range-finding experiment and two metabolic investigations, using first five and then ten percent S9 fraction, were conducted. In the second and conclusive experiment for mutagenicity, dose levels for R-8, R-8 from algae, S-8 and Swedish Biofuel consisted of 0.156, 0.313, 0.625, 1.25, 2.5, and 5 microliter/plate. The doses for Amyris were 0.0013, 0.0025, 0.005, 0.01, 0.02, 0.039, and 0.078 microliter/plate for the four Salmonella strains and 0.078, 0.156, 0.313, 0.625, 1.25, 2.5, and 5 microliter/plate for the E. coli strain. The five jet fuels, Amyris, R-8, R-8 from algae, Fischer Tropsch fuel S-8, and Swedish Biofuel, were judged to be nonmutagenic under the test conditions used in this experiment; therefore, the test substances were determined to be negative in the bacterial reverse mutation assay.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2010
- Accession Number
- ADA536581
Entities
People
- Carol E. Green
- David R. Mattie
- Edward S. Riccio
Organizations
- SRI International