F-T Jet Fuel Reverse Mutation Assay and Chromosome Aberration Test
Abstract
Two assays were performed in order to investigate the potential of Fischer Tropsch (F-T) jet fuel for its ability to induce genotoxicity. First, to determine its ability to induce gene mutations, the plate incorporation test was performed with the Salmonella typhimurium strains TA 98, TA 100, TA 102, TA 1535 and TA 1537. No toxic effects of the test item were noted in any of the five tester strains used up to the highest dose group evaluated (with and without metabolic activation). F-T jet fuel did not cause gene mutations by base pair changes or frameshifts in the genome of the tester strains used. Therefore, F -T jet fuel is considered to be non-mutagenic in this bacterial reverse mutation assay. Second, to investigate a possible potential of F -T jet fuel for its ability to induce structural chromosome aberrations in human lymphocytes in vitro, a chromosome aberration assay was carried out. During the described in vitro chromosomal aberration test and under the experimental conditions reported, the test item F-T jet fuel did not induce structural chromosomal aberrations in human lymphocyte cells. Therefore, F-T jet fuel is considered to be non-clastogenic in this chromosome aberration test.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2010
- Accession Number
- ADA536685
Entities
People
- Angela Lutterbach
- Barbara Wallner
- David R. Mattie
- Dean J. Wagner
- Margit Oppong-nketiah
- Teresa R Sterner
- Thomas E. Becker
- Wolfram Riedel
Organizations
- Henry M. Jackson Foundation for the Advancement of Military Medicine