Human Neural Cell-Based Biosensor
Abstract
Human neural progenitor cells have a strong potential for use as cell-based biosensors for environmental toxins. The overall goal of this project is to develop a human neural cell based biosensor using ArunA's neural progenitor cell line, hNP1(trademark). In this report, we detail the progress in development for the following areas: (1) neural progenitor isolation from induced pluripotent stem cells, (2) directed differentiation of progenitors into dopaminergic neurons, motoneurons and astrocytes using defined medium conditions, (3) cell-based methods to detect botulinum toxin, and (4) fluorescence based assays for proliferation, cell migration, mitochondrial function, reactive oxygen species generation and apoptosis as sensor elements.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 11, 2011
- Accession Number
- ADA538497
Entities
People
- Allan Powe
- Jamie Chilton
- Steven L. Stice