Next Generation of Advanced Laser Fluorescence Technology for Characterization of Natural Aquatic Environments
Abstract
The project research addresses our long-term goal to develop an analytical suite of the Advanced Laser Fluorescence (ALF) methods and instruments to improve our capacity for characterization of aquatic environments. The ALF technique (Chekalyuk and Hafez, 2008) sponsored in 2005-2008 by NOAA/CICEET and NSF uniquely combines high-resolution spectrally and temporally resolved measurements of the laser-stimulated emission (LSE). The real-time LSE spectral deconvolution provides assessments of key bio-geochemical variables, including chlorophyll-a (Chl-a), phycobiliprotein pigments, and chromophoric dissolved organic matter (CDOM). Three spectral types of phycoerythrin (PE) are discriminated for detection and characterization of PBP-containing phytoplankton and cyanobacteria. The spectrally-corrected pump-during-probe (PDP) measurements of variable fluorescence, Fv/Fm, yield improved assessments of phytoplankton photosynthetic capacity and biomass. An extensive series of flow-through ALF measurements in diverse water types, have demonstrated ALF utility as an informative integrated tool for aquatic research and observations. The ALF operational integration into the major oceanographic programs is currently in progress, including the California Current Ecosystem Long Term Ecological Research (CCE LTER, NSF) and California Cooperative Oceanic Fisheries Investigations (CalCOFI, NOAA).
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2010
- Accession Number
- ADA539217
Entities
People
- Alexander Chekalyuk
- Andrew Barnard
- Mati Kahru
Organizations
- Lamont–Doherty Earth Observatory