Sealing Penetrating Eye Injuries With Photoactivated Bonding
Abstract
Our goal is to develop a light-activated technology with the potential to decrease vision loss and ocular complications in warfighters sustaining penetrating eye injuries. In year 1, the scope was to establish the treatment parameters for sealing amniotic membrane over penetrating corneal lacerations (ex vivo and in vivo) and to design a light delivery system that reduces retinal radiant exposure to below established safe limits. We identified the treatment parameters (amnion patch size, dye concentration, dye staining time and the irradiance and fluence of green light) that strongly bonded Rose Bengal-stained amnion patches over corneal wounds in ex vivo rabbit eyes. Determined that 100 J/cm2 of 532 nm laser light (6.5 min irradiation) forms a seal between amnion and cornea in vivo that resists opening at intraocular pressures up to 350 mm Hg. Designed and constructed optical system for direct light-activated bonding of penetrating injuries.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2010
- Accession Number
- ADA540409
Entities
People
- Irene E. Kochevar
Organizations
- Massachusetts General Hospital