Two-Photon Excited Fluorescence from Biological Aerosol Particles

Abstract

We used a 40 MHz mode-locked 524 nm laser source to evaluate the utility of sub-picosecond excitation of fluorescence from 2-photon absorption in biological aerosols. Individual particles of biological composition, as well as other calibration particles, suspended in an inlet air flow were illuminated and measured as they passed through an optical chamber. To our knowledge, this is the first demonstration of 2-photon excited fluorescence from micron-sized particles composed of micro-organisms. We also observed a high fluorescence signal at visible wavelengths, which was not present with single-photon excitation.

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Document Details

Document Type
Technical Report
Publication Date
Sep 29, 2010
Accession Number
ADA540420

Entities

People

  • J.W. Lou
  • Jay Eversole
  • Marc Currie
  • Vasanthi Sivaprakasam

Organizations

  • United States Naval Research Laboratory

Tags

Communities of Interest

  • Counter WMD
  • Energy and Power Technologies

DTIC Thesaurus Topics

  • Biological Aerosols
  • Chemistry
  • Detection
  • Detectors
  • Dispersions
  • Laser Beams
  • Laser Induced Fluorescence
  • Lasers
  • Materials
  • Measurement
  • Molecules
  • Optical Fiber Lasers
  • Optical Materials
  • Optics
  • Particle Size
  • Scattering
  • Tryptophan

Fields of Study

  • Physics

Readers

  • Aerosol Science/Aerosol Physics
  • Optical Physics and Photonics.
  • Quantum Dot Semiconductor Device Photonics and Graphene Optoelectronic Materials and THz Physics.

Technology Areas

  • Directed Energy
  • Directed Energy - Lasers