Early Prediction of Lupus Nephritis Using Advanced Proteomics
Abstract
The purpose of this project is to identify initial biomarker patterns in SLE nephritis using screening proteomic profiling, and advanced proteomic profiling. Subject recruitment has been completed (150 children with SLE, out of which 75 have lupus nephritis). Utility of one of the biomarkers (NGAL) in predicting worsening of global and renal SLE disease activity has been validated. We found 2 proteins significantly over-expressed in Class IV vs Class V lupus nephritis by 2D gel electrophoresis: albumin fragments (25kDa) and alpha-1-B glycoprotein (60kDa). We found over 30 proteins differentially expressed in Class IV vs Class V lupus nephritis by SELDI-TOF-MS. Additional proteomic profiling studies using NMR- and MS-based metabonomics as well as LC/MS based protein profiling using Thermo LTQ FT-ICR have been initiated. Overall, these studies will identify a subset of non-invasive biomarkers that identify lupus nephritis subclasses, and predict the clinical course of the disease. The significance of such biomarkers is that they will provide novel non-invasive tools to identify patients with lupus nephritis, to risk-stratify the subjects for therapies, and to follow the efficacy of therapies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2009
- Accession Number
- ADA541314
Entities
People
- Prasad Devarajan