NF-kappaB Activity in Macrophages Determines Metastatic Potential of Breast Tumor Cells
Abstract
Both macrophages and NF-kappaB (NF-alpha B) signaling are known to be important in mammary tumorigenesis. However, the contribution of NF-alpha B signaling within the macrophages to metastatic potential has not been directly investigated in vivo. We proposed to use transgenic models in a novel combination to address this critically important question related to breast to lung metastasis. Expression of an inhibitor (DN) or an activator (IKK) of NF-alpha B can be induced by crossing either with a transgenic expressing the reverse transactivator (rtTA) protein in a specific cell type and administering doxycycline (dox) in drinking water (1). The rtTA protein has been targeted to macrophages (2). A third type of transgenics (NGL), act as an in vivo reporter of NF-alpha B activity and indicates overall inflammatory response (3). We have these transgenics and have combined them to modulate NF-alpha B activity in macrophages and to test the impact of this altered microenvironment on breast cancer metastasis to the lung.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2010
- Accession Number
- ADA541379
Entities
People
- Fiona E. Yull
Organizations
- Vanderbilt University