Intracellular Nanoparticle Aggregation as a Mechanism for Inducing Apoptosis in Breast Cancer Cells

Abstract

The current paradigm of drug delivery using nanotechnology has been focused on coupling chemotherapeutic molecules to nanoparticulate delivery systems. In this work, we propose that pH-triggered aggregation alone of nanoparticles (that are non-toxic when not aggregated) can induce death in breast cancer cells. We hypothesize that non-toxic, protein nanoparticles, when internalized by endocytosis and triggered to aggregate inside breast cancer cells, will be cytotoxic to them. We will examine (a) the extent to which pH-induced intracellular aggregation is cytotoxic, and (b) whether coupling a chemotherapeutic drug to a pH-responsive protein nanoparticle yields synergistic effects on the toxicity. Although this was a Concept Award which only funded one year of proposed research, a one-year, no-cost extension has been granted to complete the remaining tasks. This current report reflects the one-year update report.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2010
Accession Number
ADA542536

Entities

People

  • Szu-Wen Wang

Organizations

  • University of California, Irvine

Tags

DTIC Thesaurus Topics

  • Biochemical Engineering
  • Bioengineering
  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Couplings
  • Cytoplasm
  • Department Of Defense
  • Engineering
  • Materials
  • Materials Science
  • Nanomaterials
  • Nanoparticles
  • Neoplasms
  • Organelles
  • Toxicity

Readers

  • Molecular and Cellular Biochemistry
  • Nanocomposite Materials Science
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech