The Role of Central Metabolism in Prostate Cancer Progression
Abstract
We hypothesize that by enriching the diet with Omega-3 PUFAs PCa tumor progression will be significantly reduced. Patients with localized PCa will be enrolled in a randomized, doubleblinded phase-I clinical trial in which they will be given Omega-3 fish oil or control oleic acid supplements 5 weeks prior to prostatectomy. We will evaluate differential protein and phosphopeptide expression in PCa cells, obtained from laser capture microdissected tissue, by mass spectrometry. As primary endpoints, we will compare proliferation and apoptosis in tumors from each group using immunohistochemistry and DNA nick end labeling. As secondary endpoints, we will compare serum prostate specific antigen and hormone levels. We will also measure levels of dietary PUFAs in red blood cell membranes as well as FASN and PUFA metabolic products in prostate tissue using high performance liquid chromatography, mass spectrometry, and gas chromatography.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2010
- Accession Number
- ADA542541
Entities
People
- Thomas P Conrads
Organizations
- University of Pittsburgh