The Isolation and Characterization of Human Prostate Cancer Stem Cells
Abstract
The overall objective of this proposal is to develop a durable cure for lethal prostate cancer through the elucidation of the role of cancer stem cells in the pathogenesis of the disease. During the past year, we have made the following significant findings/observations: i) employing a specific feeder layer (rSVM), human prostate cancer xenograft cells from 3D structures we have termed glandoids, ii) glandoids appear to be clonal and form tumors in vivo that recapitulate the parent xenograft and iii) readily obtaining rSVM for in vitro cultures is challenging. We continue to optimize and validate our in vitro model of prostate cancer initiation to facilitate cancer stem cell discovery as well as drug targeting.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2011
- Accession Number
- ADA542728
Entities
People
- Ganesh Palappattu
Organizations
- Houston Methodist Research Institute