Human Neural Cell-Based Biosensor

Abstract

Human neural progenitor cells have a strong potential for use as cell-based biosensors for environmental toxins. The overall goal of this project is to develop a human neural cell based biosensor using ArunA's neural cell lines. In this report, we detail progress in development for the following areas: (1) neural progenitor isolation from induced pluripotent stem cells, (2) directed differentiation of progenitors into dopaminergic neurons, motoneurons and astrocytes using defined medium conditions, (3) cell-based methods to detect botulinum toxin, and (4) HTS amenable assays for proliferation, differentiation, cell migration, mitochondrial function, reactive oxygen species generation and apoptosis as sensor elements.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jun 11, 2011
Accession Number
ADA544446

Entities

People

  • Allan Powe
  • Jamie Chilton
  • Steven L. Stice

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Astrocytes
  • Beta Testing
  • Biosensors
  • Cell Line
  • Cell Movement
  • Cells
  • Chemistry
  • Contracts
  • Department Of Defense
  • Detectors
  • Electronic Mail
  • Instructions
  • Migration
  • Military Research
  • Neurons
  • Stem Cells

Fields of Study

  • Biology
  • Chemistry

Readers

  • Immunology and Pathology
  • Molecular Genetics
  • Neural Network Machine Learning.

Technology Areas

  • Biotechnology