Androgenic Regulation of White Adipose Tissue-Prostate Cancer Interactions
Abstract
During this year, we generated Glipr1 WT (wild-type) and KO (knockout) male mice, surgically castrated them, and collected the VP and epididymal WAT on days 3, 14, and 35 after castration, as outlined in the Statement of Work ([SOW], Aim 1, Tasks 1-3). We isolated RNA and performed microarray analysis to characterize genes affected by castration in WAT obtained 14 days after castration from both mouse genotypes. Analyses of all VP tissue and of WAT collected 3, 14, and 35 days after castration and studies outlined in the SOW, Aim 2, Task 1, are ongoing. Our major findings are 1) Castration induces WAT wet weight (ww) and WAT ww: body weight ratio reductions in mice of both genotypes, but with different kinetics: the reduction is delayed with Glipr1 knockout. Further, testosterone offsets the effect of castration on WAT ww 14 and 35 days after castration in both genotypes. 2) Castration induces morphologic changes in adipocytes: cells become smaller, with larger stromal compartments. Kinetics of the castration-induced WAT ww reduction differs between the two genotypes. 3) Castration induces changes in the WAT genome: we detected genes whose up- or down-regulation was Glipr1-genotype specific.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2011
- Accession Number
- ADA545293
Entities
People
- Timothy C Thompson
Organizations
- The University of Texas MD Anderson Cancer Center