Development of PCSA-Targeted Minibodies for Imaging of Prostate Cancer
Abstract
Prostate stem cell antigen (PSCA), a cell surface marker on prostate cancer cells, was used to generated humanized antibody and combined with PET imaging to image prostate tumor. To improve affinity, the humanized anti-PSCA antibody fragments were reformatted and screened for better affinity as well as better tumor imaging ability by PET. During the past year, three minibody variants were affinity-matured, and A11 appears to be the lead agent in terms of specific immunoreactivity and superior imaging contrast. In parallel, specific hPSCA expression was validated in the hPSCA-KI mouse model. Experiment testing A11 in hPSCA-KI mice is in progress, and we expect to obtain exciting results in the next few months. At the same time, crossing to prostate cancer model such as the PTEN conditional knockout is also underway, and we expect to get the compound mice by early next year.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2010
- Accession Number
- ADA545373
Entities
People
- Robert Reiter
Organizations
- University of California, Los Angeles