Screening and Selection of New Antagonists of the RING-Mediated Hdm2/Hdmx Interaction

Abstract

During the first period of the grant we have been able: 1) Produce large libraries (106 different cyclotides) of cyclotides in E. coli cells using the loop 2 of the cylotide MCoTI-I (Specific aim #1, Task 2a) 2) We have cloned, expressed and characterized a fluorogenic reporter to screen in-cell cyclotide libraries for inhibitors of the RING-mediated interaction between Mdm2/MdmX (specific aim #1, Task 1a & 1b).

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2011
Accession Number
ADA545593

Entities

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  • Julio A. Camerero

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  • University of Southern California

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