Estrogen-DNA Adducts as Novel Biomarkers For Ovarian Cancer Risk and for Use in Prevention

Abstract

The purpose of this research is to determine the association between ovarian cancer and (1) imbalances in estrogen metabolism that lead to higher levels of estrogen-DNA adducts and/or (2) genetic polymorphisms in selected enzymes that metabolize estrogens. The first year of the grant has been spent collecting urine and saliva samples from cases and controls, establishing the protocol to purify the maximum amount of DNA from saliva samples, synthesizing estrogen metabolite, conjugate and DNA adduct standards for MS/MS analysis, optimizing the UPLC-MS/MS method, and setting up a database in ACCESS to analyze data collected from the subject questionnaires. All of these analyses will be carried out in the second year of the grant.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Mar 31, 2011
Accession Number
ADA546084

Entities

People

  • Eleanor G. Rogan

Organizations

  • University of Nebraska Omaha

Tags

DTIC Thesaurus Topics

  • Biological Markers
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Databases
  • Department Of Defense
  • Epithelial Cells
  • Estrogens
  • Genetic Variation
  • Medical Personnel
  • Metabolism
  • Metabolites
  • Neoplasms
  • Ovarian Cancer
  • Questionnaires
  • Standards

Readers

  • Gulf War Illness and Chronic Multisymptom Illness in Veterans.
  • Molecular Biology and Genetics
  • Neurotoxicology

Technology Areas

  • Biotechnology