Application of Nucleic Acid-Based Tools for Monitoring Monitored Natural Attenuation (MNA), Biostimulation and Bioaugmentation at Chlorinated Solvent Sites
Abstract
Successful anaerobic bioremediation at chlorinated solvent sites relies on the presence of bacteria, such as Dehalococcoides (Dhc), capable of organohalide respiration (i.e., respiratory reductive dechlorination or "[de]chlororespiration"). Nucleic acid-based assays like the quantitative real-time polymerase chain reaction (qPCR) technique detect and enumerate Dhc in soil or groundwater samples by targeting Dhc-specific biomarker genes, including the 16S rRNA gene and the tceA, bvcA, and vcrA reductive dechlorinase (RDase) genes implicated in chlorinated ethene reductive dechlorination. The results of nucleic acid-based tests, like the qPCR approach, are expected to assist site managers and practitioners to identify sites where implementation of long-term monitored natural attenuation (MNA) will be effective; where biostimulation will achieve complete dechlorination without DCE/VC "stall"; and where bioaugmentation is required.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2010
- Accession Number
- ADA546820
Entities
People
- Carmen Lebrón
- Erik A. Petrovskis
- Frank E. Loeffler
- Janet Hatt
- Keith Henn
- Kirsti Ritalahti
Organizations
- Environmental Security Technology Certification Program