Induced Pluripotent Stem Cells as Potential Therapeutic Agents in NF1

Abstract

In this project, we are seeking to use induced pluripotent stem (iPS) cell technology as a potential therapy in neurofibromatosis 1 (NF1). In the first year, we have successfully produced iPS cells from fobroblasts from Nf1+/- mice and characterized the properties of these cells, which include growth in clusters, expression of stem cell markers, normal karyotype, and the ability to form teratomas in mice. We also have created a targeting "gene-repair" vector to replace the defective Nf1 allele in these iPS cells. Thus, we are on schedule for this project, having achieved our aims in the expected time frame.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2011
Accession Number
ADA547230

Entities

People

  • Jonathan Chernoff

Tags

DTIC Thesaurus Topics

  • Anatomy
  • Biological Sciences
  • Biomedical Research
  • Bone Marrow
  • Bone Marrow Cells
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Cells (Biology)
  • Department Of Defense
  • Diseases And Disorders
  • Epithelial Cells
  • Fibroblasts
  • Neoplasms
  • Standards
  • Stem Cells
  • Targeting

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biology

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech