Coordination of BRCA1/BARD1- and MRE11/RAD50/NBS1-Dependent DNA Transactions in Breast Tumor Suppression

Abstract

The main objective of the proposal is to understand how the Mre11-Rad50-Nbs1 complex and the BRCA1-BARD1 complex interact on DNA and coordinate DNA transactions that are critical for the maintenance of genomic stability and to prevent breast tumor development. We have started to characterize the behavior of BRAC1/BARD1 on DNA, using a single molecule approach. We have purified recombinant MRN and BRCA1/BARD1 complexes. We have shown that Whereas MRN is essential for processing DNA ends at double-strand breaks (DSBs), BRCA1 is dispensable for this function. In contrast both BRCA1 and MRN complex are required for checkpoint activation following DNA damage.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2009
Accession Number
ADA548368

Entities

People

  • Eric Greene
  • Jean Gautier

Organizations

  • Columbia University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Carrier Proteins
  • Cell Line
  • Cells
  • Chromosome Structures
  • Contrast
  • Gel Electrophoresis
  • Leading Edges
  • Maintenance
  • Molecules
  • Nanotechnology
  • Neoplasms
  • New York
  • Optical Materials
  • Proteins
  • Quantum Dots

Readers

  • Aerospace Engineering.
  • Molecular Genetics
  • Oncology (Cancer Research).