Targeting the Reactive Stroma Niche in Prostate Cancer
Abstract
Rate of prostate cancer progression is affected by the reactive stroma microenvironment. Our previous studies have shown that reactive stroma is regulated by TGF-beta pathways. The objectives of the proposed research is to assess the origin/ Ontogeny of reactive stroma in cancer and fundamental mechanisms of recruitment/activation in prostate cancer. To date, we have addressed studies proposed for each Task. We have developed an in vivo matrix trapping approach to isolate, characterize and culture reactive stromal cell recruited to Matrigel plugs. We have also developed a three dimensional co-culture model that permits co-culture of prostate carcinoma cell spheroids with prostate stromal progenitor cells. We have evaluated reactive stroma recruitment in mice that receive xenograft implants of prostate cancer cells. We have assessed the effects of TGF-beta on reactive stroma differentiation. These studies will allow us to dissect key mechanisms that mediate recruitment of reactive stroma to the tumor microenvironment and to target these mechanisms in order to inhibit tumorigenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2011
- Accession Number
- ADA548625
Entities
People
- David R. Rowley
Organizations
- Baylor College of Medicine