Green Synthesis of Phloroglucinol: Exploiting Pseudomonas fluorescens and Scale-Up
Abstract
A new generation of phloroglucinol synthesizing microbe has been evaluated using resin-based extractive fermentation at 1 L scale. Strategies to understand in vivo phloroglucinol synthase expression in this genetically engineered E. coli were examined. Improving phloroglucinol synthase activity is essential to increase the microbial phloroglucinol synthesis titer and yield. Strategies to identify novel phloroglucinol synthases were therefore explored. It is also believed that expression of phloroglucinol synthase phlD gene inside its native Pseudomonas fluorescens Pf-5 will likely deliver higher expression level and activity of this gene product. Constructing a phloroglucinol synthesizing P. fluorescens was therefore pursued.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2010
- Accession Number
- ADA548823
Entities
People
- John W. Frost