Green Synthesis of D-1,2,4-Butanetriol from D-Glucose
Abstract
To build a more robust microbe for production of D-1,2,4-butanetriol with minimal amount of byproducts formation, various experimental parameters including microbial genetic make-up, media component and fermentation reaction condition were investigated. The investigation provided an optimized E. coli biocatalyst KIT18/pWN7.126B carrying a codon-optimized Pseudomonas putida mdlC benzoylformate decarboxylase and an alcohol dehydrogenase, adhP capable of producing an upward of 35 g/L of D-1,2,4-butanetriol from D-xylose. However the process also generates byproducts, such as, 3,4-dihydroxy-D-butanol and 3,4-dihydroxy-D-butanoic acid, that are difficult to separate and purified. To circumvent this problem and eliminate the need of using xylose as a carbon source, a new two-step approach to D-1,2,4-butanetriol synthesis from glucose has been developed. The process relies on the use of biocatalyst E. coli, WY9/pWY1, for first converting D-glucose to D-xylonic acid, which is then utilized by E. coli biocatalyst DH5x/pWN6.186A, which carries a codon optimized P. putida mdlC plasmid encoding benzoylformate decarboxylase while relying on native D-xylonate transport along with native D-xylonate dehydratase and dehydrogenase activities for the synthesis of D-1,2,4-butanetriol. The two-step process has been demonstrated as capable of producing D-1,2,4-butanetriol from glucose, a much more reliable source of feedstock, successfully.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 31, 2009
- Accession Number
- ADA548856
Entities
People
- John W. Frost