Engineering Complex Microbial Phenotypes with Continuous Genetic Integration and Plasmid Based Multi-Gene Library
Abstract
Scientific and Technical Objectives: To develop tools & strategies to facilitate the development of complex phenotypes in microbial cells by combining DNA from different organisms in order to develop desirable complex traits, such as tolerance for chemicals, such as ethanol (EtOH) and 1,2,4-butanetriol (BT), which are used as "testbeds" for the proof of the proposed concept. BT is a chemical of interest to Navy for possible production by a biological process using metabolically engineered cells. Objective 1: Build complex phenotypes by accelerated evolution to generate "new" genomes in order to enable selection of desired phenotypes. The goal is to enlarge the Escherichia coli genome by selective integration of genes from strains that have desirable traits. Here, we aim to enlarge the E. coli genome using Lactobacillus plantarum genes to build cells tolerant to EtOH and BT. L. plantarum is an organism with established high tolerance to alcohols and solvents more broadly. Objective 2: Build a stress-response (or chaperone) system that can be customized for tolerance to chosen toxic chemicals, such as EtOH and BT.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2010
- Accession Number
- ADA548874
Entities
People
- Eleftherios T Papoutsakis
Organizations
- University of Delaware