Discovery of Hyperpolarized Molecular Imaging Biomarkers in a Novel Prostate Tissue Slice Culture Model
Abstract
Our goal for the first year was to optimize conditions for maintaining human prostate tissue slices (TSCs) in an NMR- compatible, 3-D tissue culture bioreactor and to verify the metabolic integrity of TSCs over time. We have successfully provided a tissue culture bioreactor and optimum culture conditions and procedures to acquire good spectral quality data from metabolically viable human prostate tissue slices. In collaboration with Drs. Kurhanewicz and Peehl, we were able to acquire non-hyperpolarized and hyperpolarized 13C MR data acquired from human tissue slices that demonstrated the metabolism of the human tissue slices accurately reflects the metabolism of the in vivo human prostate and that [1- 13C]hyperpolarized lactate will be an accurate biomarker of prostate cancer in patients.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2011
- Accession Number
- ADA549047
Entities
People
- Sabrina Ronen
Organizations
- University of California, San Francisco