Toll-Like Receptor-9-Mediated Invasion in Breast Cancer
Abstract
The focus of this research is to discern the structural and biophysical features of small deoxyoligonucleotides that have significant biological properties including the inducement of Toll-like receptor 9 (TLR9) in the mediation of cellular invasion. Cell invasion (metastasis) is a significant problem in the control and treatment of breast cancer. Recent research from our laboratory has demonstrated enhanced cellular invasion in the MDA-MB-231 breast cancer cells by ODN-M362, a 25-base single-stranded CpG-containing deoxyoligonucleotide. Although the mechanism(s) for this induction is unknown, our studies reveal key insights into the structural and sequence requirements for DNA activation of this cellular invasion process. The deoxyoligonucleotides that are most effective in eliciting an invasion response have been shown to adopt stable structural motifs including stem-loops or hairpins or G-quadruplex structures. Sequence modifications have been designed to probe base sequence, structure, and stabilities that are required for initiating TLR-9 mediated cellular invasion. Our results demonstrate that these small deoxyoligonucleotides and the stability of their secondary structures play a pivotal role in eliciting the TLR9-induced invasion process.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2011
- Accession Number
- ADA549125
Entities
People
- David E. Graves
Organizations
- University of Alabama