Discovery of Hyperpolarized Molecular Imaging Biomarkers in a Novel Prostate Tissue Slice Culture Model
Abstract
We have successfully achieved the goals of the first aim, to optimize conditions for maintaining human prostate tissue slices (TSCs) in an NMR- compatible, 3-D tissue culture bioreactor and to verify the metabolic integrity of TSCs over time. Of particular importance, the results of the first year of funded studies demonstrate the feasibility of maintaining both the pathologic and metabolic integrity of benign and malignant human tissue cultures in the NMR compatible bioreactor for 32 hours. Moreover, nonhyperpolarized and hyperpolarized 13C MR data acquired from human tissue slices demonstrated that the metabolism of the human tissue slices accurately reflects the metabolism of the in vivo human prostate and that [1-13C]hyperpolarized lactate will be an accurate biomarker of prostate cancer in patients.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2011
- Accession Number
- ADA549212
Entities
People
- John Kurhanewicz
Organizations
- University of California, San Francisco