Cancer-Associated PCNA as a Potential Biomarker for Breast Cancer
Abstract
Two separate isoforms of the marker of cellular proliferation, proliferating cell nuclear antigen (PCNA) have recently been identified. The acidic isoform, as opposed to its basic counterpart has been associated with neoplasia. This cancer associated PCNA (caPCNA) can now be identified by a specific antibody, caPCNAab, demonstrated to distinguish between normal breast tissue and invasive breast cancer by IHC. Additionally, this rabbit polyclonal antibody can also detect circulating caPCNA in the serum of patients with breast cancer (unpublished data). These data suggest that caPCNA may be a potential biomarker of breast cancer and the antibody to caPCNA, caPCNAab, may serve as a detector of breast cancer. The clinical utility of this antibody to cancer specific PCNA is untested. We have proposed to utilize this newly identified antibody, in a recently created ELISA format, to distinguish the cancer associated form of PCNA (caPCNA) from the blood of both normal volunteers and breast cancer patients to evaluate whether there is a statistically significant detectable difference.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2010
- Accession Number
- ADA549224
Entities
People
- Ramona Swaby
Organizations
- Fox Chase Cancer Center