Global Characterization of Protein Altering Mutations in Prostate Cancer
Abstract
The identification of recurrent, protein-altering genetic alterations is frequently the means by which a given gene is initially implicated in tumor biology. Our specific aims are as follows: (1) Carry out the genome-wide identification of nonsynonymous mutations in a limited number of prostate metastases using second-generation technologies for targeted capture and sequencing; (2) Evaluate the mutational histories of individual mutations within the progression of the cancer in which it was observed, and to assess the prevalence of candidate cancer genes observed here in prostate cancer. (3) Perform integrative analyses of somatic mutation with gene expression and copy number change data collected on the same samples. During this funding period we have: (i) completed the identification and quality assessment of the prostate cancer tissues/samples that will be used for the duration of the project; (ii) completed DNA isolation for 204 samples/cases of prostate cancer that will be used for the project; (iii) transferred the first group of DNAs to the Shendure lab for Exome analysis; (iv) participated in interpreting the Exome sequence data for the first 23 samples. These studies have produced the exciting and novel result identifying a hyper-mutated prostate cancer phenotype; (v) completed copy number analyses for 70 prostate cancers; (vi) identified, and confirmed, recurrent point mutations in several genes and genetic pathways; (vii) initiated network-based integrated molecular analyses of advanced prostate cancers that includes copy-number variation and transcript profiles.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2011
- Accession Number
- ADA549530
Entities
People
- Peter S Nelson
Organizations
- Fred Hutchinson Cancer Center